این مقاله انگلیسی در 10 صفحه در سال 2016 منتشر شده و ترجمه آن 12 صفحه میباشد. کیفیت ترجمه این مقاله ویژه – طلایی ⭐️⭐️⭐️ بوده و به صورت کامل ترجمه شده است.

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• فهرست مطالب:

چکیده
مقدمه
مواد و روشها
ساختار تیمار
ارزیابی
تحلیل آماری
نتیجه و بحث
درصد گیاهان گل دهنده
تعداد گلها به ازای هر گیاه
ارتفاع گیاه
تعداد پیازها
محیط پیاز
وزن تر و خشک پیازها

• بخشی از ترجمه:

وزن تر و خشک پیازها
وزن تر پیازها تنها تحت تاثیر شکل برداشت ساقه قرار می گیرد. حذف جوانه های موجود در بخشهای رویشی (برگها و ساقه ها) موجب تولید پیازهایی می شود که میانگین وزن تر آنها 17.39 گرم، 62% و 54% بیشتر از مقادیر حاصل به دست آمده برای برداشت کامل ساقه بودند (6.53 گرم) و برای شرایطی که در برداشت ساقه 10 سانت در خاک باقی می ماند (8.09 گرم) (جدول 4).تغییرات در وزن/ ماده خشک پیازها که در واقع تحت تاثیر اثر متقابل همه تیمارها قرار می گیرند (جدول 59) در گیاهانی بود که حاصل رشد یازهایی به 1.9 تا 2.5 سانت و 3.2 تا 3.8 سانت در قطر بودند که در معرض 25 روز بهاره سازی قرار گرفته بودند، صرفنظر از آن شکل برداشت اختلاف معنی داری در ماده خشک تولیدی ایجاد نکرد. گیاهان حاصل از پیازهای با اندازه متوسط (2.5 تا 3.2 سانت) در واقع، وقتی در معرض 25 روز بهاره سازی قرار می گیرند، پیازهایی با ماده خشک بیشتر تولید می کنند که در این زمان برداشت شامل حذف جوانه های گلدهی است.

• بخشی از مقاله انگلیسی:

Fresh and dry bulb weights

The fresh bulb weights was influenced only by the form of stem harvest. Excision of the floral button leaving the vegetative parts (leaves and stems) enabled the production of bulbs whose average fresh weight was 17.39 g, 62% and 54% higher than the values obtained for the full harvest of the stem (6.53g) and for the stem harvest leaving 10 cm of stem in the soil (8.09g) (Table 4). Variations in the dry mass/weight of the produced bulbs were in turn influenced by the interaction of all treatments (Table 5), where plants grown from bulbs with 1.9 to 2.5cm and 3.2 to 3.8cm in diameter, subjected to 25 days of vernalization, regardless of the form of harvest showed no significant differences in the dry matter produced. Plants from intermediate sized bulbs (2.5 to 3.2cm) in turn, produced bulbs with higher dry mass when exposed to 25 days of vernalization, when the harvest included the removal of floral buds.

دانلود رایگان مقاله انگلیسی          خرید ترجمه فارسی مقاله

نوشته دانلود ترجمه مقاله اثر بهاره سازی با تولید گل شاخه بریده زنبق (۲۰۱۶) (ترجمه ویژه – طلایی ⭐️⭐️⭐️) اولین بار در ترجمه فا پدیدار شد.

مشخصات مقاله انگلیسی (PDF)
سال انتشار	2015
تعداد صفحات مقاله انگلیسی	12 صفحه با فرمت pdf
نوع مقاله	ISI
نوع ارائه مقاله	ژورنال
رشته های مرتبط با این مقاله	کشاورزی و زیست شناسی
گرایش های مرتبط با این مقاله	علوم سلولی و مولکولی، علوم گیاهی و فیزیولوژی گیاهان زراعتی
چاپ شده در مجله (ژورنال)	مجله تجربی گیاه شناسی – Journal of Experimental Botany
کلمات کلیدی	Arabidopsis، ساعت شبانه روزی، CO، گلدهی، FT، دوره نوری
کلمات کلیدی انگلیسی	Arabidopsis – circadian clock – CO – flowering – FT – photoperiod
ارائه شده از دانشگاه	فیزیولوژی سلول مولکولی، دانشکده زیست شناسی، دانشگاه بیلیفلد، آلمان
نمایه (index)	MedLine – Scopus – Master journals – JCR
نویسندگان	Mikael Johansson and Dorothee Staiger
شناسه شاپا یا ISSN	ISSN 1460-2431
شناسه دیجیتال – doi	https://doi.org/10.1093/jxb/eru441
ایمپکت فاکتور(IF) مجله	5.091 در سال 2018
شاخص H_index مجله	209 در سال 2019
شاخص SJR مجله	2.560 در سال 2018
شاخص Q یا Quartile (چارک)	Q1 در سال 2018
بیس	است ✓
مدل مفهومی	دارد ✓
پرسشنامه	ندارد ☓
متغیر	ندارد ☓
رفرنس	دارد ✓
کد محصول	9683
لینک مقاله در سایت مرجع	لینک این مقاله در نشریه Oxford Journals
نشریه Oxford Journals

نوشته دانلود ترجمه مقاله تعامل بین فتو پریود و ساعت شبانه روزی – Oxford Journals 2015 اولین بار در ترجمه فا پدیدار شد.

• بخشی از ترجمه:

اما ما در اينجا براي اولين بار گزارش خواهيم كرد كه غلظت پايين آدنين سولفات (1.0 mg l_1) براي P.indicum موثر است.ريشه ها بعد از انتقال شاخه ها به محيط كشت حاوي غلظت پايين IBA (0.5 mg l_1) القا شد(84%)( Thulaseedharanو Vaidyanathan 1990).در عوض، غلظت زياد IBA باعث ريشه دهي (100%) شد.گياهچه هاي ريشه دار با موفقيت به خاك داراي 96%ميزان ماندگاري انتقال داده شد. گياهان احياء شده هيچ تغيير آشكاري درمورفولوژي يا ويژگي ها رشدي در مقايسه با گياهان دهندۀ مربوطه نشان ندادند.واكنش گلدهي تا حد زيادي به غلظت موارد استعمال شده بستگي داشت. لزوم تنظيم كنندۀ رشدگياهي براي گلدهي آزمايشگاهي متغير بود.در اين مطالعه،محيط هاي كشت حاوي اكسين چه به تنهايي و چه در تركيب با BAP باعث گلدهي در كشت هاي كالوز P.indicum شد.يافته هاي مشابه توسط Tepferو ديگران (1966) و Peeters و ديگران (1994) مشاهده شد. ضرورت اكسين براي القا و نمو گل در چند گياه از قبيل Torenia ، Vigna radiate، Vigna mungo، Pisum sativum  و Perilla frutescens گزارش شده است.در بررسي حاضر، IBA باعث حداكثر تعداد جوانه هاي مولد(تناسلي ) شد(شكل 2E و F).قابليت گلدهي با افزايش غلظت IBA مورد استفاده در شاخه هاي احياء شده كه از محيط كشت BAP+IAA+آدنين سولفات جدا شده بودند افزايش يافت.تأثر موثر وكارامد پورين ها و پيريميدن ها بر القاي گل توسط Chailakhyan و ديگران (1961) در مورد Perilla nankinensis  كشت شده در آزمايشگاه نيز گزارش شده است.مشاهدات ما با گزارش فوق مبني بر اينكه سطح مناسب آدنين و BAP براي گلدهي مفيد فايده است مطبقت دارد.مشخص شد كه گلدهي در آزمايشگاه نيز تحت تأثر سطوح و نسبت دو مولفۀ اصلي يعني كربوهيدرات ها ومواد معدني رخ مي دهد.حضور منبع كربوهيدرات يك الزام يوبيكيتس(ubiquitous )براي نمو و القاي معتبر گل ها در آزمايشگاه ميباشد.گلدهي  P. indicum به حضور منبع كربوهيدرات بستگي دارد.در بررسي حاضر،مشخصشد كه ساكارز بهترين گزنه براي القاي جوانۀ گل م باشد. فراواني بالاي گلدهي بعد از تكميل محيط كشت با 3% (w/v) ساكارز مشاهده شد.اين نتيجه با گزارشات پيشين در Lycopersicon esculantum، سيب زميني، V.mungo، P. sativum،و Gentiana trifolia مطابقت دارد.براساس فرضيۀ انحراف مادۀمغذي گلدهي ،نسبت C/N در جوانه در طي القاي گل افزايش ميابد.در بررسي حاضر، محيط كشت MS با مكمل 3% (w/v) ساكارز و تنظيم كننده هاي رشد گياه ،چند شاخۀ گلدار توليد كرد.اين نتيجه نشان مي دهد كه نسبت C/N براي گلدهي مهم است.مطالعت زيادي تأثير سطح نيتروژن بر گلدهي آزمايشگاهي را گزارش كرده اند.

• بخشی از مقاله انگلیسی:

Pentanema indicum Ling. belongs to the family Asteraceae (Compositae). It is a female antifertility drug used by tribes in Bihar state of India. The plant is an annual and is distributed throughout India, ascending up to an altitude of 1800 m in Himalayas, Pakistan, Burma, China, Sri Lanka, Thailand and Africa. It is an erect, rigid, leafy herb 1–3 ft in height. The roots are tough, woody and externally pale brown in colour. Stem terete, striate, with many pubescent branches. Leaves are variable in size, sessile, oblong–lanceolate, acute, entire or serrulate, rough or scrabid with short appressed hairs on both sides. Ray florets 12–24, much longer than involcure and disc florets scanty. Many compounds have been isolated from P. indicum namely, sesquiterpene– vicolides, monoterpenediol–vicodiol and thymol esters (Saradha Vasanth et al. 1990; Mossa et al. 1997), cis–cis germacranolide (Sawaiker et al. 1998), 4,5,6-trihydroxy- 4-7-dimethoxy flavone (Krishnaveni et al. 1997). Vicolide D showed abortifacient and antifertility activities (Alam et al. 1989). The hexane soluble fraction showed antiviral activity against Ranikhet virus (Chowdhury et al. 1990). In vitro flowering offers a unique system in the study of molecular basis and hormonal regulation of flowering. There is also a possibility of using in vitro flowering in ecological and genetic studies. It is a modern area of research and has great potential in plant breeding programmes. In vitro propagation of medicinal plants could help in raising disease free health clones in a large scale for extraction of pure drug. To date only one report available on regeneration for this important medicinal plant (Thulaseedharan and Vaidyanathan 1990) reported callus induction and plant regeneration in Vicoa indica. However, regeneration using apical bud and nodal explants derived from mature plants is not available. Therefore, the efficiency of apical bud and nodal explants to regenerate P. indicum plants has been explored in the present study. In addition, we have demonstrated a callus mediated plant regeneration system and in vitro flowering. Actively growing shoots were used as the explants source. The shoots were separated from the mother plants, defoliated and cut into 4–6 cm pieces. The explants (leaf and stem) were surface sterilized with 70% (v/v) ethanol for 60 s and 0.1% (w/v) mercuric chloride for 10 min, washed four times with sterile distilled water. Leaf and stem segments 0.5–1.0 cm in length, were prepared and inoculated on Murashige and Skoog (MS) medium (Murashige and Skoog 1962) supplemented with plant growth regulators. The pH of the medium was adjusted to 5.7 with 0.1 N NaOH or 0.1 N HCl before autoclaving at 1.06 kg cm
2 and 121°C for 15 min. All culture media contained 3% (w/v) sucrose and solidified with 0.8% (w/v) agar. Basal medium supplemented either with BAP or BAP combined with IBA were used for callus induction from leaf and stem explants. The callus induction rate (explants with callus formation) was determined after 30 days. After four weeks, the calli proliferated upon the leaf and stem segments. The calli were transferred on MS medium supplemented with different concentrations and combinations of BAP and IAA to induce plant regeneration. Regenerated shoots were dissected out, cut into shoot tips and single nodal segments, and subcultured on MS medium containing different concentration of an auxin and a cytokinin. Multiplication of these shoots was tested in the same media or in media supplemented with adenine sulfate (1.0 mg l
1 ). After four weeks of culture plantlets were transferred to fresh medium containing same composition of ingredients. Individual shoots, which were 3–4 cm long were excised from the shoot clump and transferred to half strength MS medium containing IAA and IBA (0.5, 10, 1.5 and 2.0 mg l
1 ). All cultures were incubated at 252°C under cool fluorescent light (3000 Lux; 16-h photoperiod). After 35 days the rooted plantlets were removed from the tubes, washed thoroughly with sterile distilled water to remove traces of agar and planted in small plastic pots filled with mixture of sterile soil, sand, vermiculite (1 : 1 : 1). The potted plants were irrigated with MS basal salts solution (1/4 strength) devoid of sucrose and myo-inositol every four days for three weeks.

دانلود رایگان مقاله انگلیسی

خرید ترجمه فارسی مقاله

نوشته دانلود ترجمه مقاله ریزتکثیر و گلدهی آزمایشگاهی در گیاه پنتانما ایندیکوم لینگ اولین بار در ترجمه فا پدیدار شد.

فهرست مطالب:

چکیده
۱ مقدمه
۲ مواد و روش ها
۳ نتایج
۱ ۳ اثرات قطع برگچه(کاسبرگ) بر پارامترهای رشد گیاه
۲ ۳ اثرات قطع برگ چه بر ظهور غلاف
۳ ۳ تاثیر بر روی ریزش غلاف
۴ ۳ تاثیرات بر قطع گیاهچه بر تعداد غلاف رسیده
۵ ۳ تاثیر تیمار قطع برگچه بر توزیع گره ها و غلف ها بین ساقه اصلی و شاخه های رسیده
۶ ۳ تاثیر قطع برگچه بر تعداد بذر بالقوه در هر غلاف، تعداد بذر واقعی در هر غلاف و سقط بذر
۷ ۳ تاثیر قطع برگچه بر اندازه بذر
۸ ۳ تاثیرات قطع برگچه بر عملکرد بذر
۴ بحث
۱ ۴ قطع برگچه و پارامتر های رشد گیاه
۲ ۴ قطع برگچه و ظهور غلاف
۳ ۴ قطع برگچه و ریزش غلاف
۴ ۴ قطع برگچه ها و تعداد غلاف رسیده
۵ ۴ قطع برگچه و الگوی توزیع گره و غلاف بین ساقه ها و شاخه های رسیده
۶ ۴ قطع برگچه و سقط بذر
۷ ۴ قطع برگچه و اندازه بذر
۸ ۴ قطع برگچه و عملکرد
۵ نتیجه گیری

بخشی از ترجمه:

 بیشتر تغییرات عملکرد در گیاهان سویا (GlycinemaxL. Merrill) مربوط به تغییرات در  تعداد غلاف و بذر می باشد. تعدا غلاف حاصل ظهور  و ریزش غلاف در حالی که تعداد بذر حاصل تعداد بذر بالقوه در هر غلاف و سقط بذر است. با این حال  تنظیم فیزیولوژیک این فرایند ها به خوبی درک و مطالعه نشده است. به منظور بررسی نقش تغییرات پس از گل دهی در اندازه مخزن و ساختار تاج پوشش بر روی ظهور غلاف، قطه و ریزش آن و  سقط بذر در سویا، یک ازمایش میدانی صورت گرفت. دو ژنوتیپ سویای DM48و A7409(به ترتیب گروه های بلوغ  ۴ و ۷) مورد استفاده قرار گرفتند. تیمار های  قطع برگچه(کاسبرگ) متشکل از تیمار قطع صفر(L0)، یک (L1)  و یا دو(L2)  برگچه(کاسبرگ)  از هر برگ سه برگچه(کاسبرگ) ای موجود بودند.قطع برگچه(کاسبرگ) ها دو بار اعمال شدند: نخست در هنگاتم  گلدهی کامل و  اندکی بعد از آغاز مرحله تولید بذر. سرعت رشد گیاه(CGR)، شاخص سطح برگ(LAI)، جذب نور(LI) و سرعت رشد نسبی برگ طی دوره هایی تعیین شدند  که در ان   مولفه ها و اجزای عددی ثابت می شوند. برای دوره بین نخستین و دومین قطع برگچه(کاسبرگ)، CGR در میان تیمارهای L در هر دو ژنوتیپ بدون تغییر باقی ماند زیرا  کاهش LAI با افزایش  سرعت آزیمیلاسیون برگ های باقی مانده جبران شد. نخستین مرحله قطع برگچه(کاسبرگ) ها سرعت رشد نسبی برگ و تعدا  غلاف های ظهور یافته را افزایش داد و این افزایش ها  ارتباط معکوسی با LAI باقی مانده در هر دو ژنوتیپ داشتند. به علاوه رابطه معکوس بین LAI و PI در LAI پایین و بالاتر از سطح بحرانی ثابت باقی ماند و ارتباطی با CGR یاLI نداشت.تعداد غلاف  ریخته شده با  افزایش قطع برگچه(کاسبرگ) طی اولین و مهمترین دوره قطع در هر دو ژنوتیپ افزایش یافت و درصد ریزش غلاف ارتباط مستقیم با سرعت رشد بذر در واحد سطح برگ طی دوره ریزش و قطع داشت. سقط و نارسی بذر ارتباط معکوس با شاخص سطح برگ بعد از دومین دوره تیمار قطع برگچه(کاسبرگ) داشت. تنها بالاترین سطح قطع برگچه(کاسبرگ) ها (یعنی L2) توانست باعث کاهش اندازه برگ در هر دو ژنوتیپ شود. اگرچه ریزش غلاف، سقط بذر و اندازه بذر  شاخص های  مربوط به قدرت و توانایی آزمیلاسیون تاج پوشش برگی هستند، این مسئله حاکی از ان است که رشد سریع و بسته شدن سریع تاج پوشش ممکن است اثر منفی بر ظهور غلاف در سویا داشته باشد. ب تا آنجا که ما می دانیم این مطالعه اولین  موردی است که  نشان می دهد که  تعداد غلاف های ظاهر شده ارتباط معکوس با شاخص سطح برگ در تاج پوشش های  سویا دارد.

 ۱ مقدمه

اجزاء اصلی عملکرد سویا شامل  تعداد بذر (بذر در متر مربع) و اندازه بذر (میلی گرم /بذر) می باشند. بهبود عملیات زراعی یا بهره وری ژنتیکی می تواند  باعث افزایش عملکرد در آینده از طریق افزایش تعداد  و اندازه بذر یا هر دو اجزاء شود. تعداد غلاف   ارتباط تنگاتنگی با  تعداد بذر دارد زیرا  تعداد واقعی بذر در هر غلاف حاکی از  تنوع و تغییرات زیست محیطی اندک است( اگلی ۱۹۹۸). قابلیت دسترسی به آزمیلات ها از فاکتورهای اصلی است که  تنظیم کننده تغییرات تعداد بذر و غلاف می باشد(بورد و تان ۱۹۹۵، جیانگ و اگلی ۱۹۹۳). تغییرات در  شاخص سطح برگ، جذب نور، یا سرعت رشد گیاه که شاخصی است که به عنوان براورد کننده فتوسنتز استفاده می شود ناشی از اختلافات در  تعداد بذر و غلاف بوده اند((Herbert ) ، Harville, 1994; Board and Tan, 1995)

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abstract Much of the yield variation in soybean (Glycine max L. Merrill) crops is related to changes in pod and seed number. Pod number is the result of pod initiation and pod abscission while seed number is the result of potential seed per pod and seed abortion. However, the physiological regulation of these processes is not well understood. A field experiment was conducted to investigate the role of post-flowering changes in source size and canopy structure on pod initiation, pod abscission and seed abortion in soybean. Two soybean genotypes: DM48 and A7409 (maturity groups IV and VII, respectively) were used. Leaflet removal treatments (L) consisted of removing none (L0), one (L1) or two (L2) lateral leaflets of every developed trifoliate leaf present. Leaflet removals were applied twice: the first at full bloom and the second shortly after the beginning seed stage. Crop growth rate (CGR), leaf area index (LAI), light interception (LI), and relative leaf growth rate, were determined during the periods in which numerical components are established. For the period between the first and the second leaflet removal, CGR remained unchanged among L treatments in both genotypes because LAI reductions were compensated through an increase in the net assimilation rate of the remaining leaves. The first leaflet removal increased the relative leaf growth rate and the number of pods initiated (PI) and these increases were inversely related to the remaining LAI in both genotypes. Moreover, the inverse relationship between LAI and PI was sustained at LAI below and above critical (i.e., LAI for 95% LI) and was not related to CGR or LI. The number of pod abscised also increased with the level of leaflet removal during the first and main abscission period in both genotypes and the percentage of pod abscission was directly related to the seed growth rate per unit leaf area during the abscission period. Seed abortion was inversely related to LAI after the second leaflet removal. Only the highest level of leaflet removals (i.e., L2) was able to reduce seed size in both genotypes. Whereas pod abscission, seed abortion and seed size could be related to indicators of canopy assimilatory capability pod initiation was not, suggesting that other physiological mechanism/s operate in the regulation of pod initiation. In addition, our results suggest that early (i.e., at flowering) canopy closure may negatively impact pod initiation in soybean. To the best of our knowledge, this study is the first to document that the number of initiated pods is inversely related to LAI in soybean canopies. © 2010 Elsevier B.V. All rights reserved. 1. Introduction The primary components of soybean yield are seed number (seed/m2) and seed size (mg/seed). Improvements in agronomic practices or genetic gains could increase future yields by increasing seed number, seed size, or both components. Pod number has been shown to be highly associated with seed number because the actual number of seeds per pod shows low environmental variation (Egli, 1998). Assimilate availability has been considered to be the main factor that regulates pod and seed number changes . Variations in leaf area index (LAI), light interception (LI), or crop growth rate (CGR), which is an index usually used as an estimator of canopy photosynthesis, have been associated with differences in pod and seed number (Herbert and Litchfield, 1984; Ramseur et al., 1985; Board and Harville, 1994; Board and Tan, 1995). Linear relationships between LI or CGR measured from full flowering to the beginning of seed growth, and pod or seed number at maturity were then considered as evidence that pod and seed number are source-limited (Egli and Zhen-wen, 1991; Board et al., 1995; Board and Harville, 1998). These models explain well the relationship between pod or seed number and CGR when CGR is abruptly reduced through defoliation or shading, but the association becomes weak when additional genetic or environmental factors are involved (Egli and Bruening, 2000). On the other hand, there are situations where CGR increases significantly while seed number remains unchanged, which indicate that higher CGR does not necessarily mean higher Pod number is determined by pod initiation and pod abscission, and seed number is determined by the potential number of seeds per pod and seed abortion. These components are sequentially established and partially overlap during development. They may also have different physiological and/or environmental requirements for reaching their maximum potentials. In addition to assimilates, other environmental and/or internal signals may affect soybean pod initiation and abscission (Heindl and Brun, 1983; Myers et al., 1987; Kokubun and Honda, 2000) as well as seed number and filling (Morandi et al., 1988, 1990). In this study we investigated the effects of changes in post- flowering source size and canopy structure on pod initiation, pod abscission, and seed abortion in soybean. Evidence will be presented showing that the number of pods initiated was inversely related to LAI while it was not related to CGR or LI, suggesting that other physiological mechanisms, not directly related to assimilatory capability, were involved in the regulation of pod initiation in soybean canopies. 2. Materials and methods A field experiment was conducted at the research field of the Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina (33◦01 S, 60◦52 W) during the 2001–2002 growing season. The emergence date was December 1st, 2001. Soybean genotypes were DM48 (Maturity Group (MG) IV) and A7409 (MG VII), both of which had an indeterminate growth habit. These genotypes were commercial varieties that have been extensively sown in Argentina. Genotypes were over-seeded and thinned to a final seed density of 32 and 26 plants/m2 for DM48 and A7409, respectively. Individual plots were 6 m long and 3.2 m wide (eight rows with 0.40 m between rows). The plots were irrigated when necessary to avoid water deficits. Pests, diseases and weeds were permanently controlled during the experiment. Phenological stages were defined according to Fehr and Caviness (1977). Full bloom (R2), beginning seed (R5), beginning maturity (R7), and full maturity (R8) occurred at 42, 66, 121 and 130 days after emergence (DAE), respectively, for DM48 and at 66, 87, 128 and 140 DAE, respectively, for A7409. Leaflet removal treatments consisted of removing none (L0), one (L1) or two (L2) lateral leaflets of every trifoliate leaf at R2 and again shortly after R5. Thus, the canopy structure was modified by homogeneous removal of leaflets of all developed leaves that were present at the time of treatment. The LAI was estimated using regression functions that related leaf weight with LAI data from another experiment conducted in the same field, which used the same genotypes and leaflet removal treatments. Coefficients of determination (R2) for these functions ranged from 86% to 99% and were statistically significant in all cases (P < 0.05). A radiation sensor (pyranometer), an air temperature sensor and a bucket rain gauge were connected to a LI-COR LI-1200 Data set recorder to measure incident global solar radiation, temperature and rainfall. Sensor instruments were placed near the experimental plots. Statistical differences between climatic variables were determined by t-tests using standard errors. The experimental design was a split plot with three replicates. Genotypes (G) were themain plots, and they were arranged in a randomised, complete block design. Leaflet removal treatments were randomised within each main plot and applied two times (T1 and T2). For DM48, leaflet removals were performed at phenological stages R2 (T1) and R5 + 7 d (T2), which were 42 and 73 DAE, respectively. For A7409, leaflet removals were performed at R2 + 4 d (T1) and R5 + 13 d (T2), which were 70 and 100 DAE, respectively. The period from T1 to T2 was named Per1, and the period from T2 to R7 was named Per2. Light interception (photosynthetic active radiation, PAR) was measured immediately after T1 and T2 with a LI-COR Line Quantum sensor (LI-COR, Lincoln, NE) connected to an LI-1000 data logger. Percentage of light interception was determined in each plot from readings made above the canopy and at ground level. Measurements were taken on clear day, between 1130 and 1400 h solar time. Line quantum was placed on the ground diagonally between the plot rows. In each plot, two readings were taken at different random positions. The LI values on the other dates were estimated by a function developed from the relationship between LI and LAI for DM48 (R2 = 0.97, P < 0.0001) and A7409 (R2 = 0.95, P < 0.0001). The extinction coefficient of the canopy (k) was obtained from the Lambert-Beer’s law, such that, k = −ln(Ii/I0)/LAI, where ln means natural logarithm, Ii is PAR at soil level and I0 is PAR above the canopy (Gardner et al., 1985). Between T1 and T2, four destructive samples of 0.25 m2 were taken for both genotypes. Between T2 and R7, three destructive samples were taken for DM48, and two destructive samples were taken for A7409. For each sample, all plants were separated into stems, leaves (petioles plus leaflets), pods and seeds (when present). Samples were dried to a constant weight at 60 ◦C in a forced-air dryer, and dry matter was expressed as grams per square meter (land basis). The number of pods was counted in all sampled plants at 54, 72, 83, 97 and 111 DAE for DM48 and at 81, 87, 94, 103 and 115 DAE for A7409. A pod was counted when it was visible (≥2 mm). The number of pods initiated (PI) was defined as the maximum number of pods in the flush minus the minimum number of pods before the flush. The number of pods abscised (PA) was defined as the maximum number of pods in the flush minus the minimum number of pods after the flush. Total dry matter (TDM) per land area during the R5 to R7 period was increased to account for the photosynthate requirement of seed production [2 g of photosynthate required to produce 1 g of seed dry weight (Sinclair and de Wit, 1975)]. Total dry matter and LAI were regressed against time (Hunt and Parsons, 1981) to obtain the following indices: CGR [g/m2 (land area)/d], NAR [net assimilation rate, g/m2 (leaf area)/d] and relative leaf growth rate [m2/m2 (leaf area)/d]. Linear, quadratic and cubic components of each regression equation were successively tested for significance and included in the equation if they significantly reduced the residual sum of squares. Significant differences were determined by t-tests using standard errors calculated by the regression program. Seed growth rate (SGR) was calculated as the slope of the regression function of increased seed dry matter over time during the linear seed-growth period. The date of initiation of linear seed growth was estimated by dividing the origin ordinate of the regression function by SGR. At maturity, a 1-m2 area was harvested by cutting the plants at ground level. Harvested plants were separated into main stems, branches, seeds and pod walls. Seed size (mg/seed) was calculated as the mean dry weight of 480 randomly sampled seeds. Maturity seed number (seed/m2) was calculated as seed yield (g/m2) divided by seed size. Maturity pod number (pod/m2) was calculated as the final seed number divided by the actual seeds per pod at maturity. A sample of 10 plants was used to determine the branch number, the main stem and branch node number and the number of pods in the main stem and branches. The potential seed number per pod was established by counting the number of pods with 2 (loc2), 3 (loc3) and 4 (loc4) loculi and then resolving the following equation: [(loc2 × 2) + (loc3 × 3) + (loc4 × 4)/total pod number]. A loculus was counted if a remnant of seed structure was visible in a partially or fully developed pod loculus. Potential seed number was determined by the potential seed number per pod multiplied by the pod number at maturity. Seed abortion was calculated as the actual seed number divided by the potential seed number.

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