دانلود رایگان ترجمه مقاله گونه جدید Microcyclospora rumicis در ترشک مواج (سال 2011)

Microcyclospora rumicis، یک گونه جدید در ترشک مواج از ایران

Microcyclospora rumicis, a new species on Rumex crispus from Iran

1 Introduction

During the study of alternative weed hosts for Cercospora beticola Sacc., a causal agent of cercospora leaf spot of sugar beet in Northern Iran, a species of the genus Microcyclospora J. Frank et al. was isolated from Rumex crispus showing leaf spot symptoms in the Talesh region (Guilan province). Microcyclospora, with M. pomicola J. Frank et al. designated as type species, was recently segregated from Pseudocercospora Speg. s. str. based on phylogenetic and morphological differences (Frank et al. 2010). Currently the three species known in Microcyclospora are associated with sooty blotch and flyspeck (SBFS) blemishes on the surfaces of pomaceous fruits, specifically apples (Colby 1920; Frank et al. 2010). Here we describe a new Microcyclospora species from leaf spots on Rumex crispus that differs morphologically from the three other known species of the genus.

Materials & methods

Conditions of isolation, culture, and observation

Leaf samples of Rumex crispus showing leaf spot were collected from Rainforest Mountains in the Talesh region, Guilan province, in northern Iran near the Caspian Sea. Single conidial isolates were established directly from symptomatic curly dock leaves according to Bakhshi et al. (2011). A mass of conidia scraped from the lesion using a sterile inoculation needle under a stereomicroscope was floated in 10 ml sterile distilled water and spread on 2% malt extract agar (MEA; Fluka, Germany). After plates were incubated in a slanted position overnight, germinated conidia were transferred to new MEA plates, and cultures were incubated in the dark at 25 °C. After 30 days of incubation, samples from the colony were mounted on glass slides in clear lactic acid for microscopic examination. Thirty measurements were made for each microscopic structure, and 95% confidence intervals were derived for the measurements with extreme values given in parentheses. Line drawings were prepared using a BX41 light microscope (Olympus, Japan) equipped with a drawing tube; photos were captured using a Leica camera system. Colony colors on MEA and oatmeal agar (OA; Gams et al. 2007) [surface and reverse] were determined after one month at 25 °C in the dark. Nomenclature and descriptions were deposited in MycoBank. The holotype voucher and an ex-type culture are conserved in CCTU, the culture collection housed at the Plant Protection Department, Agriculture Faculty, University of Tabriz, Iran.